RT-qPCR is arguably the most sensitive and specific technology available for evaluating endogenous gene expression. With the advent of one-step reagents that enable qPCR directly from cell lysis, improvements in detection, and the Echo liquid handler, low cost, high-throughput RT-qPCR is now achievable. Using the Echo liquid handler, these assays can be miniaturized to nanoliter total reaction volume while guaranteeing data integrity with no cross contamination. Additionally, with no tips to interfere, re-screening is not required. With the seamless addition of automated plate handling, the Access workstation transforms the Echo platform into a walk-away solution tailored for gene expression— cell lysates direct to PCR in one step.
Key Benefits:
- One-step gene expression at volumes as low as 300 nL, with industry standard reagents and detection platforms
- High-throughput gene expression screens using more relevant cell models including primary cells
- Miniaturize 384-well qPCR assay volumes, or utilize 1536-well qPCR to mazimize throughput
- Access workstation automates the entire workflow
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